Pros and cons of immunochemical and enzymatic method in the diagnosis of Wilson's disease.
نویسندگان
چکیده
BACKGROUND Immunochemical method of measuring Ceruloplasmin (Cp) levels for the diagnosis of Wilson's disease has replaced enzymatic method for the main reason of being more sensitive and quantitative. SETTINGS AND DESIGN In this study, we compared both the methods for various factors such as sensitivity, specificity and the time consumed in the diagnosis of Wilson's disease. MATERIALS AND METHODS Serum samples from patients (n=33) with a provisional diagnosis of Wilson's disease were analyzed for Cp levels by enzymatic copper oxidase method and immunochemical method using polyclonal antibodies specific to Cp embedded in agar. STATISTICAL ANALYSIS Pearson's regression analysis was performed to compare the two methods. RESULTS The mean Cp obtained by immunochemical method is 5.87 mm +/- 1.17 and by enzymatic method, it is 0.37 (Optical Density) +/- 0.136. Pearson's Regression analysis of the measurements showed a good correlation with an 'r' value of 0.765 between the two methods. CONCLUSION A good correlation indicated that these two tests are comparable and thus both these methods can be used together for a definitive and better diagnosis of Wilson's disease.
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عنوان ژورنال:
- Indian journal of medical sciences
دوره 60 9 شماره
صفحات -
تاریخ انتشار 2006